Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endocytosis-dominated membrane area decrease requires Rab5 protein in rat melanotrophs.

Simon Sedej1, Marjan Rupnik, Robert Zorec

  • 1Laboratory of Neuroendocrinology-Molecular Cell Physiology, Institute of Pathophysiology, Medical Faculty, University of Ljubljana, Slovenia.

Annals of the New York Academy of Sciences
|September 13, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Brief mechanical pulses induce sustained intracellular L-lactate production in astrocytes.

Frontiers in cellular neuroscience·2026
Same author

Norepinephrine Couples Astrocytic Metabolism to mTOR-Driven Protein Synthesis.

Journal of neurochemistry·2026
Same author

Brain Bioenergetics in Aging: Neurovascular and Neurometabolic Coupling and Fuels: 15th International Conference on Brain Energy Metabolism.

Journal of neurochemistry·2026
Same author

Author Correction: Curing the brain: in search for new astrocyte-specific therapies.

Experimental & molecular medicine·2026
Same author

Curing the brain: in search for new astrocyte-specific therapies.

Experimental & molecular medicine·2026
Same author

Dopamine-Induced L-Lactate Production in Cortical Astrocytes Cross-Reacts with β<sub>1</sub>-Adrenoceptor-Mediated cAMP Signalling.

Aging and disease·2026

Rab5 protein is essential for regulating membrane surface area changes during endocytosis in rat pituitary cells. Blocking Rab5 inhibits endocytosis-dependent membrane capacitance decrease and affects exocytosis-stimulated membrane capacitance increase.

Area of Science:

  • Cell Biology
  • Membrane Trafficking
  • Endocrinology

Background:

  • Eukaryotic cells internalize macromolecules via endocytosis, a process requiring Rab5 protein.
  • The specific role of Rab5 in plasma membrane surface area dynamics during endocytosis remains unclear.
  • Rat melanotrophs, pituitary cells, serve as a model to investigate Rab5's function.

Purpose of the Study:

  • To determine if Rab5 protein is necessary for membrane surface area changes in rat melanotrophs.
  • To elucidate Rab5's role in regulating endocytosis and exocytosis in these cells.

Main Methods:

  • Immunocytochemistry was used to confirm the presence of Rab5 protein in melanotrophs.
  • Electrophysiological membrane capacitance measurements were employed to assess membrane surface area dynamics.

Related Experiment Videos

  • Microinjection of specific Rab5 antibodies was used to inhibit Rab5 function.

    • Main Results:

    • Rab5 protein was detected in rat melanotrophs.
    • At low intracellular calcium, Rab5 inhibition blocked the endocytosis-driven decrease in membrane capacitance.
    • At high intracellular calcium, Rab5 inhibition did not alter the steady-state membrane capacitance increase but accelerated its rate, suggesting endocytosis inhibition.

    Conclusions:

    • Rab5 protein plays a crucial role in regulating membrane surface area changes in rat melanotrophs, particularly in endocytosis.
    • Rab5 activity influences the balance between endocytosis and exocytosis at the plasma membrane.
    • These findings contribute to understanding the molecular mechanisms of membrane trafficking in pituitary cells.