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Decreased mRNA transcripts of M-phase promoting factor and its regulators in the testes of infertile men

Yung Ming Lin1, Yen Ni Teng, Chia Ling Chung

  • 1Department of Urology, National Cheng Kung University, College of Medicine, Tainan, Taiwan.

Human Reproduction (Oxford, England)
|September 13, 2005
PubMed
Abstract

Insights

Decreased M-phase promoting factor (MPF) and regulator mRNA levels in testes are linked to male infertility and failed sperm retrieval. These findings highlight the importance of MPF in human spermatogenesis.

Area of Science:

  • Reproductive Biology
  • Molecular Endocrinology

Background:

  • M-phase promoting factor (MPF), composed of Cyclin B and Cdc2, regulates cell cycle entry into M phase.
  • MPF activity is modulated by Cdc25 phosphatases and Wee1 kinase.
  • Investigating MPF and regulator transcript levels in infertile men's testes is crucial for understanding spermatogenic failure.

Purpose of the Study:

  • To quantify mRNA transcript levels of MPF (Cyclin B, Cdc2) and its regulators (Cdc25 family, Wee1) in the testes of infertile men.
  • To correlate these transcript levels with testicular phenotypes and sperm retrieval outcomes.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qPCR) was used to measure mRNA levels.
  • Analysis included testes from 37 azoospermic patients.

Main Results:

  • Significantly lower mRNA transcript levels of CDC2, CCNB1, CCNB2, CDC25A, CDC25C, and WEE1 were observed in patients with spermatogenic failure.
  • CDC2 mRNA levels positively correlated with CCNB1 and CCNB2 mRNA levels.
  • Higher transcript levels of CDC2, CCNB1, CCNB2, CDC25C, and WEE1 were found in patients with successful sperm retrieval compared to those with failed retrieval.

Conclusions:

  • Reduced mRNA transcripts of MPF and its regulators are implicated in human spermatogenic dysfunction.
  • These molecular changes may contribute to male infertility and impact sperm retrieval success.

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