Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Tissue microarrays.

Ronald Simon¹, Martina Mirlacher, Guido Sauter

¹Department of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Methods in Molecular Medicine

|September 15, 2005

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Correction to: MMR Deficiency is Homogeneous in Pancreatic Carcinoma and Associated with High Density of Cd8-Positive Lymphocytes.

Annals of surgical oncology·2022

Same author

Cytokeratin 7 and cytokeratin 20 expression in cancer: A tissue microarray study on 15,424 cancers.

Experimental and molecular pathology·2022

Same author

Metabolic Syndrome Is Associated with Impaired Survival after Surgery for Pancreatic Neuroendocrine Tumors.

Neuroendocrinology·2022

Same author

VPRBP Functions Downstream of the Androgen Receptor and OGT to Restrict p53 Activation in Prostate Cancer.

Molecular cancer research : MCR·2022

Same author

PITX1 Is a Regulator of TERT Expression in Prostate Cancer with Prognostic Power.

Cancers·2022

Same author

Combination of Biochemical and Cytological Findings for Better Diagnosis in Pleural Effusions.

Advances in experimental medicine and biology·2022

Same journal

Erratum to: Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination.

Methods in molecular medicine·2015

Same journal

Methods for cancer gene therapy using tumor suppressor genes.

Methods in molecular medicine·2014

Same journal

Suppression of the human carcinoma phenotype by an antioncogene ribozyme.

Methods in molecular medicine·2014

Same journal

Methods for the use of stromal cells for therapeutic gene therapy.

Methods in molecular medicine·2014

Same journal

Methods for adenovirus-mediated gene transfer to synovium in vivo.

Methods in molecular medicine·2014

Same journal

Methods for gene transfer to synovium.

Methods in molecular medicine·2014

See all related articles

Tissue microarray (TMA) technology enables rapid, standardized, and cost-effective in-situ analysis of numerous clinical tissue samples for evaluating candidate genes in diseases like cancer.

Area of Science:

Oncology
Genetics
Pathology

Background:

High-throughput screening identifies numerous genes implicated in cancer and other diseases.
Evaluating these candidate genes requires in-situ analysis of many clinical tissue samples.

Purpose of the Study:

To introduce and highlight the utility of tissue microarray (TMA) technology for efficient candidate gene evaluation.
To emphasize the standardization, speed, and cost-effectiveness of TMA in large-scale tissue analysis.

Main Methods:

Tissue microarray (TMA) technology involves analyzing minute tissue samples (0.6 mm) from up to 1000 tissues on a single slide.
Standard in-situ methods like immunohistochemistry, FISH, and RNA in-situ hybridization are applicable to TMAs with minimal protocol changes.

Related Experiment Videos

Main Results:

TMAs allow simultaneous analysis of multiple tissues with identical reagent batches, ensuring high standardization.
This approach significantly increases the speed and cost efficiency of analyzing large numbers of clinical samples.

Conclusions:

TMA technology is a powerful tool for the in-situ evaluation of candidate genes in a high-throughput manner.
TMAs facilitate large-scale validation studies, accelerating the discovery of genes involved in disease.