Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Binding of mycoplasmas to solid phase adsorbents.

Susan Szathmáry1, Nandani Rajapakse, Ibolya Székely

  • 1Clarigen Inc, Carlsbad, CA, USA.

Acta Veterinaria Hungarica
|September 15, 2005
PubMed
Summary

Chromatographic resins effectively removed mycoplasmas from biological fluids. This method rapidly reduces mycoplasma contamination in cell culture and aids in concentrating mycoplasmas for vaccine production.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and validation of an LNA-based multiplex RT-qPCR assay for differentiating Betaarterivirus europensis (PRRSV-1), Betaarterivirus americense (PRRSV-2), and the highly pathogenic L8 lineage of PRRSV-2.

Veterinary journal (London, England : 1997)·2026
Same author

Pre-Pulse Inhibition of an escape response in adult fruit fly, Drosophila melanogaster.

Translational psychiatry·2026
Same author

Molecular Diversity of Aquatic Pseudomonas spp. With Inhibitory Activity Against Saprolegnia Water Mould (Oomycota).

Journal of fish diseases·2025
Same author

Description of Saprolegnia velencensis sp. n. (Oomycota), a novel water mold species from Lake Velence, Hungary.

PloS one·2024
Same author

Pre-Pulse Inhibition of an escape response in adult fruit fly, <i>Drosophila melanogaster</i>.

Research square·2024
Same author

FRL and DAAM are required for lateral adhesion of interommatidial cells and patterning of the retinal floor.

Development (Cambridge, England)·2023

Area of Science:

  • Biotechnology
  • Microbiology
  • Separation Science

Background:

  • Mycoplasma contamination is a significant issue in cell culture, impacting research and biopharmaceutical production.
  • Current methods for mycoplasma removal can be time-consuming or ineffective.
  • Mycoplasmal cell membranes possess unique lipid structures and adhesion molecules exploitable for capture.

Purpose of the Study:

  • To evaluate the efficacy of various chromatographic resins for mycoplasma capture.
  • To assess the potential of this technology for mycoplasma removal from cell culture components and concentration for vaccine production.

Main Methods:

  • Testing of chromatographic resins (ActiClean Etox, ClarEtox, Heparin-Actigel, Sulfated Hiflow, SulfEtox) for mycoplasma capture.
  • Evaluation of resin performance in Phosphate Buffered Saline (PBS) and Fetal Bovine Serum (FBS).

Related Experiment Videos

  • Quantification of mycoplasma reduction using log-based measurements.

    • Main Results:

    • All tested resins demonstrated efficient reduction of mycoplasma concentrations.
    • Mycoplasma levels were reduced by 3-8 logs within minutes.
    • Effective mycoplasma removal was observed in both PBS and FBS.

    Conclusions:

    • Chromatographic resins offer a rapid and efficient method for mycoplasma removal.
    • This technology is suitable for purifying tissue culture components, such as serum.
    • The method can be applied to concentrate mycoplasmas for vaccine production purposes.