Related Experiment Video
Updated: Aug 16, 2026
An In Vitro Hemodynamic Loop Model to Investigate the Hemocytocompatibility and Host Cell Activation of Vascular Medical Devices
Published on: August 21, 2020
[Cytotoxicity study of heparin coated polyvinyl chloride material]
Jian Yang1, Dinghua Yi, Jincheng Liu
1Center of Cardiovascular Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710033, China. yangjian1212@hotmail.com
Abstract:
In this study, heparin ionically coated polyvinyl chloride (PVC) material was prepared by heparin-benzalkonium chloride complex (Group A), heparin-benzalkonium bromide complex (Group B) and heparin-polyethyleneimine compound (Group C). Cytotoxicity evaluation was conducted by direct cell contact evaluation and MTT colorimetry method. The results showed Group A and Group B caused L-929 cells to die out while Group C showed good compatibility with cells. The OD levels of Group A and B were lower than that of the Group C in MTT test. Method A and method B of heparin coating had remarkable cytotoxicity, while method C had little cytotoxicity and could be further studied for clinical use.
