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[Effects of triptolide on anti-angiogenesis by reducing expression of urokinase plasminogen activator]
Yi Ding1, Jiancheng Zhang, Lijun Hou
1Department of Pathophysiology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China.
Abstract:
In order to unveil the anti-angiogenic mechanism of triptolide, we investigated the effects of triptolide on the proliferation, migration, angiogenesis and u-PA expression of cultured HUVECs. MTT assay found that triptolide could inhibit the proliferation of HUVECs, and three-dimensional culture system revealed that triptolide could curb the migration of HUVECs. The chick embryo chorioallantoic membrane (CAM) test showed that triptolide could inhibit angiogenesis. Real time quantitive RT-PCR showed the expression of u-PA of HUVECs was down-regulated by triptolide. Therefore, triptolide may inhibit the proliferation and migration of endothelial cell by way of reducing the expression of u-PA. These findings may conduce to the elucidation of the anti-angiogenic mechanism of triptolide.
