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Updated: Aug 16, 2026
A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana
Published on: June 30, 2023
[Usefulness and practical use of an automatic counter for scattering pollen in spring]
Toru Imai1, Osamu Nohara, Tomohiko Endo
1Department of Otorhinolaryngology, St. Luke's International Hospital. imaitoru@luke.or.jp
Background:
In this decade some automatic pollen counters have been developed and showed them useful in some reports. However, the results of automatic counting have not been established.
Method:
We have compared scattering cedar and cypress pollen counts with Durham pollen samplers and a newly developed automatic pollen counter (KH-3000) in the spring from 2000 to 2004 in Tokyo.
Results:
The relationship between the data of the automatic counter and that of a Durham sampler method is so good that this automatic counter seems useful. The best relationship between two methods was seen from late February to early April in the season of high amounts of pollens scatters. The ratio of pollen counts measured with automatic counter and Durham sampler was about 3.5 on average in the period of enough cedar and cypress pollen dispersion. The avoidance of pollens seems possible with real-time pollen information because reliability of automatic counting is highly evaluated when enough pollens scatter.
Conclusion:
In future, the standardization of automatic counting should be necessary for the accurate evaluation of scattering pollens.
