Related Experiment Video
Updated: Aug 16, 2026

Generation of a RIP1 Knockout U937 Cell Line Using the CRISPR-Cas9 System
Published on: April 11, 2025
The role of Rac1 and Rac2 in bacterial killing
Adeline Loo Yee Koh1, Chun Xiang Sun, Fei Zhu
1Faculty of Dentistry, University of Toronto, Toronto, Canada.
Abstract:
The small Rho guanosine triphosphatases (GTPases) Rac1 and Rac2 have distinct roles in regulating neutrophil chemotaxis; however, little is known about their possible unique roles in mediating bacterial killing. To elucidate the relative roles of Rac1 and Rac2 in regulating neutrophil-mediated bacterial killing, we utilized the previously described mice model in which mouse neutrophils are deficient in either Rac1, Rac2, or both isoforms. We demonstrate here that while both Rac isoforms are required for normal neutrophil chemotaxis and bacterial killing, they have non-overlapping roles in bacterial phagocytosis and NADPH oxidase function.
Related Concept Videos
CRISPR and crRNAs
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
Cell Polarization by Rho Proteins
Gene Regulation in Microbial Communities: Quorum Sensing
Regulation of Bacterial Virulence
Bacterial Toxins
Intracellular Movement of Viruses and Bacteria

