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Updated: Aug 16, 2026
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
Glial fibrillary acidic protein is expressed in the aged rat olfactory epithelium
Min Kyu Kim1, Joo Hyun Park, Bum Sun Kwon
1Department of Anatomy, Seoul National University College of Medicine, Seoul, South Korea.
Conclusion:
Our findings suggest that the aging process induces changes in the phenotype of olfactory supporting cells in the rat.
Objective:
To investigate age-related changes in the expression of astroglial intermediate filament proteins in the olfactory supporting cells of the rat.
Material And Methods:
The expression of nestin and glial fibrillary acidic protein (GFAP) in the olfactory epithelium (OE) of young (3 months) and aged (25 months) Sprague-Dawley rats was compared using Western blotting and immunohistochemistry.
Results:
Western blot analysis showed nestin expression only in the young OE, whereas GFAP was detected only in the aged OE. Immunohistochemistry showed that GFAP was localized in the olfactory supporting cells of the aged OE, with regional differences.
