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Biodegradation of azo dyes by genetically engineered azoreductase
Jing Wang1, Bin Yan, Ji-Ti Zhou
1School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116023, China. wangjing1967@sohu.com
Abstract:
A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1.1737. The enzyme, with a molecular weight of 18.7 kD, was efficiently expressed in Escherichia coli and its biodegradation characteristics for azo dyes were investigated. Furthermore, the reaction kinetics and mechanism of azo dyes catalyzed by the genetically engineered azoreductase were studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.
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