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Updated: Aug 12, 2026
Assaying for Inorganic Polyphosphate in Bacteria
Published on: January 21, 2019
Analysis of phosphate-accumulating organisms cultivated under different carbon sources with polymerase chain
Shui-Li Yu1, Ya-Nan Liu, Guo-Lin Jing
1School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
Abstract:
To investigate the microbial communities of microorganisms cultivated under different carbon sources, three sequencing batch reactors were operated. They were supplied with sewage, glucose and sodium acetate as carbon sources respectively and showed high phosphorus removal performance. The results of denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction-amplified (PCR) 16S rDNA fragments demonstrated that beta-protebacteria, Actinomyces sp. and gamma-protebacteria only exited in 1 # reactor. The microbiological diversity of 1 # reactor exceeded the other two reactors. Flavobacterium, Bacillales, Actinomyces, Actinobacteridae and uncultured bacteria (AF527584, AF502204, AY592749, AB076862, AJ619051, AF495454 and AY133070) could be detected in the biological phosphorus removal reactors.
