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Heat-sensitive lambda repressors retain partial activity during bacteriophage induction.

M Lieb

    Journal of Virology
    |October 1, 1979
    PubMed
    Summary
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    Heat-inducible lambda phage (N-cIts) induction was studied. Certain heat-sensitive repressors (cI) showed partial activity even at high temperatures, affecting phage replication and gene expression.

    Area of Science:

    • Bacteriophage biology
    • Molecular genetics
    • Microbial genetics

    Background:

    • Bacteriophage lambda integrates into the host genome as a prophage.
    • Heat-inducible lambda prophages (cIts) can be triggered to enter the lytic cycle upon temperature elevation.
    • The lambda cI repressor controls the switch between lysogenic and lytic cycles.

    Purpose of the Study:

    • To investigate the kinetics of beta-galactosidase synthesis and progeny phage release in response to heat induction.
    • To compare the efficiency of induction using different heat-sensitive lambda cI mutants.
    • To determine if partial repressor activity affects prophage induction.

    Main Methods:

    • Superinfection of lac- lysogens with lambda trp/lac N+cI- phage.
    • Heat treatment of lysogens to induce prophage.

    Related Experiment Videos

  • Measurement of beta-galactosidase synthesis.
  • Quantification of progeny phage release.

    • Main Results:

    • Beta-galactosidase appeared earlier and was synthesized more rapidly in lysogens with lambda N-cIts2 or lambda N-cIts16 mutants compared to lambda N-cIts857.
    • The cI857 repressor retained partial activity even at 45°C.
    • Lysogens with other N-cIts mutants showed only partial derepression at 43°C.
    • Partial derepression of lambda early transcription is sufficient for N+ prophage induction.

    Conclusions:

    • The activity of heat-sensitive lambda repressors varies among different mutants.
    • Even partial derepression of lambda early transcription can lead to prophage induction.
    • The efficiency of phage induction is dependent on the specific cI mutant's repressor activity.