Related Experiment Video
Updated: Aug 16, 2026
Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance
Published on: March 7, 2025
Distribution of fluoroquinolone MICs in Helicobacter pylori strains from Korean patients
Jung Mogg Kim1, Joo Sung Kim, Nayoung Kim
1Department of Microbiology and Institute of Biomedical Science, Hanyang University College of Medicine, Seoul, Korea. jungmoog@hanyang.ac.kr
Objectives:
The aim of this study was to assess the prevalence rate of primary fluoroquinolone resistance in Helicobacter pylori isolates from Korean patients over the past 16 years.
Methods:
One hundred and thirty-five strains of H. pylori (34 strains in 1987, 36 in 1994 and 65 in 2003) were isolated from antral gastric mucosal biopsy specimens. The determination of MICs for the H. pylori isolates of ciprofloxacin, levofloxacin and moxifloxacin was examined by using the serial 2-fold agar dilution method. DNA sequences of the gyrA gene in fluoroquinolone-resistant strains were determined.
Results:
The distribution of fluoroquinolone MICs (ciprofloxacin, levofloxacin and moxifloxacin) shifted to higher concentrations during 1987-2003. All of the levofloxacin- or moxifloxacin-resistant strains were resistant to ciprofloxacin. Sequence analysis in fluoroquinolone-resistant strains showed point mutation of the gyrA gene at A272G and G271A, indicating mutations of the codon Asp-91 in the fluoroquinolone-resistance-determining region of the DNA gyrase.
Conclusions:
These results suggest that resistance to fluoroquinolones has been increasing in the Korean population and the resistance is most likely mediated through point mutation in gyrA.
Insights
Primary fluoroquinolone resistance in Helicobacter pylori is increasing in Korean patients. This rise is linked to specific mutations in the gyrA gene, impacting treatment effectiveness.
Area of Science:
- Microbiology
- Genetics
- Pharmacology
Background:
- Helicobacter pylori infection is a global health concern.
- Fluoroquinolones are crucial antibiotics for treating H. pylori infections.
- Understanding antimicrobial resistance patterns is vital for effective treatment strategies.
Purpose of the Study:
- To determine the prevalence of primary fluoroquinolone resistance in Korean H. pylori isolates over 16 years.
- To investigate the genetic mechanisms underlying fluoroquinolone resistance in H. pylori.
Main Methods:
- Analysis of 135 H. pylori strains isolated from Korean patients between 1987 and 2003.
- Determination of minimum inhibitory concentrations (MICs) for ciprofloxacin, levofloxacin, and moxifloxacin.
- DNA sequencing of the gyrA gene in fluoroquinolone-resistant strains.
Main Results:
- A significant increase in fluoroquinolone MICs was observed from 1987 to 2003.
- All levofloxacin- or moxifloxacin-resistant strains were also resistant to ciprofloxacin.
- Fluoroquinolone resistance was associated with specific point mutations (A272G and G271A) in the gyrA gene, affecting codon Asp-91.
Conclusions:
- Fluoroquinolone resistance in H. pylori is rising in the Korean population.
- Point mutations in the gyrA gene are the primary mechanism mediating this resistance.
- These findings highlight the need for monitoring resistance trends and developing alternative treatment options.
More Related Videos
06:40Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance
Published on: July 28, 2023
04:56Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis
Published on: May 16, 2025
Related Concept Videos
01:16Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy
01:28Inhibitors of Bacterial DNA Synthesis