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Related Experiment Videos

Staining properties of deepithelialized human amniotic membrane.

Ganesha R Kandavel1, Roy S Chuck

  • 1Department of Ophthalmology, University of California, Irvine, CA, USA.

Cornea
|September 15, 2005
PubMed
Summary

Fluorescein sodium and lissamine green dyes offer temporary staining for human amniotic membrane (HAM) visualization during surgery. Other dyes like rose bengal and trypan blue show persistent staining, potentially limiting their clinical utility.

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Area of Science:

  • Ophthalmology
  • Tissue Engineering
  • Surgical Innovation

Background:

  • Human amniotic membrane (HAM) is a valuable surgical graft due to its regenerative properties.
  • The transparency and thinness of HAM present challenges for intraoperative handling and visualization.
  • Dye staining is explored to enhance the visibility of HAM during surgical procedures.

Purpose of the Study:

  • To evaluate the in vitro staining properties and duration of 5 vital dyes on deepithelialized human amniotic membrane (HAM).
  • To determine the optimal dyes for intraoperative visualization of HAM based on staining persistence and fading characteristics.

Main Methods:

  • Deepithelialized HAM samples were stained with varying concentrations of 5 vital dyes: indocyanine green, fluorescein sodium, lissamine green, rose bengal, and trypan blue.

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  • Staining intensity was assessed using a x10 operating microscope at multiple time points up to 24 hours, including intermediate evaluations after rinsing and resuspension.
  • The duration of staining for each dye and concentration was recorded.

    • Main Results:

    • All tested vital dyes initially stained the deepithelialized HAM.
    • Fluorescein sodium (at 0.1% and 0.25%) and lissamine green (at 0.1% and 0.5%) demonstrated fading staining within 120-210 minutes.
    • Indocyanine green, rose bengal, and trypan blue exhibited persistent staining at 24 hours across all tested concentrations.

    Conclusions:

    • Fluorescein sodium and lissamine green show potential for intraoperative HAM visualization due to their transient staining properties.
    • Rose bengal, trypan blue, and indocyanine green may be less suitable for clinical application due to prolonged staining.
    • The choice of dye concentration is critical for achieving desired staining duration and facilitating surgical handling of HAM.