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Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

Covalent immobilization of recombinant fusion proteins with hAGT for single molecule force spectroscopy

Stefan K Kufer1, Hendrik Dietz, Christian Albrecht

  • 1Lehrstuhl für Angewandte Physik, Sektion Physik, Ludwig-Maximilians-Universität München and Center for NanoScience, Amalienstrasse 54, 80799 Munich, Germany.

European Biophysics Journal : EBJ
|September 15, 2005
PubMed

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Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...