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Determination of active components in Cynanchum chinense R. Br. by capillary electrophoresis
Huitao Liu1, Yuan Gao, Ketai Wang
1Department of Applied Chemistry, Yantai University, Yantai 264005, People's Republic of China. liuht@lzu.edu.cn
Abstract:
A method for the determination of 7-O-alpha-l-rhamnopyranosyl-kaempferol-3-O-beta-d-glucopyranoside (GL) and 7-O-alpha-l-rhamnopyranosyl-kaempferol-3-O-alpha-l-rhamnopyranoside (RH) in the traditional Chinese herb Cynanchum chinense R. Br. by capillary electrophoresis has been developed. With botate buffer (30 mmol/L, pH 9.50) as running buffer and an applied voltage of 20 kV, the compounds were completely separated within 6 min and detected at UV 254 nm. The correlation coefficients of the calibration curves for GL and RH were 0.9990 and 0.9992, respectively, over the concentration ranges (15.0-1000.0 and 12.0-1000.0 microg/mL), and the recoveries were from 91.4 to 107.1%.
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