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[Determination of cholesteryl palmitate in Oviductus Ranae by HPLC]
Yong-sheng Wang1, Da-cheng Jiang, Qin Meng
1Jilin University, Changchun 130021, China.
Objective:
To develop a new method for the determination of cholesteryl palmitate in Oviductus Ranae.
Method:
A HPLC method was set up, using Zorbax Silica column and cyclohexane-diethyl ether (40:1) as mobile phase with a flow rate of 1.0 mL x min(-1), and the UV detection wavelength was 203 nm.
Result:
The calibration curve was linear over the range of 0.60-8.92 microg (r = 0.9997), the average recovery of the method was 98.4%. RSD 1.8% (n = 6).
Conclusion:
The results showed that method was reliable and accurate.
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