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Updated: Aug 12, 2026
ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
Published on: October 23, 2009
Development of immunoassay for detection of meat and bone meal in animal feed
Shin-Hee Kim1, Tung-Shi Huang, Thomas A Seymour
1Department of Nutritional Sciences, College of Human Environmental Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA. kimshin@okstate.edu
Abstract:
An immunoassay system was developed for efficient detection of prohibited meat and bone meal (MBM) in animal feed. Monoclonal antibodies (MAbs) were raised against bovine smooth muscle autoclaved at 130 degrees C for 20 min. Among the 1,500 supernatants of hybridoma cells screened, MAbs 3E1, 1G3, and 3E10 were selected and characterized in this study. The first set of MAbs produced, 3E1 and 1G3, had stronger reactivity against MBM than against smooth muscle that was heat treated at 90 degrees C for 10 min. However, reactivity gradually increased against smooth muscle that was autoclaved at 130 degrees C for up to 1 h. The enzyme-linked immunosorbent assay for detection of MBM in animal feed was optimized with the MAb 3E10 because of its superior performance. MAb 3E10 diluted to 100-fold was used to differentiate bovine MBM from that of other species in ingredients used for commercial animal feeds and could detect down to 0.05% MBM mixed in animal feed.
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