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Bifidobacterium-selective isolation and enumeration from chicken caeca by a modified oligosaccharide
S N Thitaram1, G R Siragusa, A Hinton
1Agricultural Research Service, United States Department of Agriculture, Russell Research Center, Athens, GA 30605, USA.
Aims:
To determine the efficacy and selectivity of an acidified, antibiotic-selective, oligosaccharide-containing media for enumerating Bifidobacterium spp. from chicken caeca samples.
Methods And Results:
Transoligosaccharide propionate agar medium (TOS) modified by addition of mupirocin (50 microg ml-1) and glacial acetic acid (1%, v/v), did not inhibit the growth of bifidobacteria compared with the control media yet inhibited the growth of Lactobacillus acidophilus, Lactobacillus gallinarum, Lactobacillus helveticus and Streptococcus gordonii.
Conclusions:
Addition of mupirocin (50 microg ml-1) and glacial acetic acid (1%, v/v) to TOS (TOS-AM50), is an effective selective medium for isolation and enumeration of Bifidobacterium spp. from chicken caeca samples.
Significance And Impact Of The Study:
The development of an intestinal bifidobacteria-selective media contributes to the study of probiotics and prebiotics in poultry and potentially other species.
