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Published on: August 20, 2018
Construction of transgenic Bacillus mucilaginosus strain with improved phytase secretion
1The Keylaboratory of Bioactive Material, Ministry of Education; Life Science College, Nankai University, Tianjin, China. mgl@nankai.edu.cn
Aims:
To construct a transgenic Bacillus mucilaginosus strain to increase the secretion capability of a wild-type isolate of B. mucilaginosus D4B1 to hydrolyse phytate phosphorus, which can be used as a microbial fertilizer in field application.
Methods And Results:
We constructed a phytase secreting expression vector pSP43 with a mini-Tn5 transposon and a Aspergillus fumigatus phytase expression cassette. The vector pSP43 was successfully transferred into the wild-type B. mucilaginosus using the particle bombardment method, and three transgenic strains with a stable copy of phytase expression cassette integrated into the chromosome of the B. mucilaginosus by Tn5 transposition were selected. The phytase activity of the engineered strains increased 36-46-fold when compared with the wild-type strain of D4B1.
Conclusions:
The A. fumigatus phytase gene can be expressed under the direction of p43 promoter in B. mucilaginosus. The expression protein is secreted extracellularly and newly constructed strains showed a high phytase activity.
Significance And Impact Of The Study:
A transgenic Bacillus strain by the particle bombardment method was constructed.
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