Related Experiment Video
Updated: Aug 15, 2026
Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation
Published on: July 6, 2019
Blunted inflammatory response to lipopolysaccharide endotracheal instillation due to thermal preconditioning
A Di Filippo1, S Rinaldi, C Pascente
1Section of Anaesthesia, Department of Critical Care, University of Florence, Viale Morgagni 85, 50124 Florence, Italy. aledanibatibeba@hotmail.com
Aim:
Heat shock proteins (HSPs), induced by thermal stress, make the cells more resistant to following stress. We aimed to verify if rats heating reduces the production of TNF-alpha in a model of acute respiratory distress syndrome (ARDS).
Methods:
We divided 34 Wistar rats in Group A and Group B. The rats of Group A received thermal shock and tracheal instillation of lipopolysaccharide (LPS), the rats of Group B only tracheal instillation of LPS. All the rats have been then subdivided in 6 subgroups sacrificed at different times after instillation of LPS: A30 and B30 at 30 min, A90 and B90 at 90 min and A180 and B180 at 180 min. We dosed the serum concentration of TNF-alpha and the values of every subgroup have been compared with the Student t-test.
Results:
The serum concentration of TNF-alpha in the subgroup A90 resulted significantly lower than in the subgroup B90.
Conclusions:
The exposure to thermal stress resulting in the production of HSPs reduced the concentration of TNF-alpha in this model of ARDS.
More Related Videos
04:10Non-Invasive Endotracheal Administration of Lipopolysaccharide to Induce Acute Lung Injury in Rodents
Published on: December 5, 2025
12:27The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice
Published on: April 2, 2014