Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Computer program for analyzing donor photobleaching FRET image series.

Gergely Szentesi1, György Vereb, Gábor Horváth

  • 1Department of Biophysics and Cell Biology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|September 16, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Augmenting CAR T cell functionality and metabolism through CD39 downtuning.

Molecular therapy. Oncology·2026
Same author

[Long-COVID syndrome and lung-specific abnormalities following COVID-19].

Orvosi hetilap·2026
Same author

[The role of obesity and weight reduction in obstructive sleep apnea].

Orvosi hetilap·2026
Same author

Hierarchical Functionalisation of UiO-66(Zr)-NH<sub>2</sub> with Cysteine, PEG, and SARS-CoV-2 Spike RBD to Facilitate ACE2 Receptor Targeting in Model Cells.

Nanomaterials (Basel, Switzerland)·2026
Same author

Ten years of translational innovation in HER2-targeted immune cell therapy - A comprehensive review of the work of the University of Debrecen Cell and Molecular Therapy Research Group.

Magyar onkologia·2026
Same author

Evaluation of the diagnostic value of the Modified Evan's Blue Dye Test for assessing aspiration in tracheostomized critically ill patients: A systematic review and meta-analysis.

PloS one·2026

A new software tool analyzes photobleaching fluorescence resonance energy transfer (pbFRET) data, enabling pixel-by-pixel calculation of energy transfer values. This facilitates proximity measurements of labeled macromolecules in biological samples.

Area of Science:

  • Spectroscopy and microscopy
  • Biophysics
  • Molecular biology

Background:

  • Photobleaching fluorescence resonance energy transfer (pbFRET) is a microscopy technique for measuring macromolecule proximity.
  • pbFRET analysis requires pixel-by-pixel calculation of bleaching time constants from image series.
  • Existing software lacks the capability to process the extensive data required for pbFRET.

Purpose of the Study:

  • To develop and introduce new evaluation software for analyzing pbFRET data.
  • To address the limitations of existing software in processing pbFRET image series.
  • To enable efficient and comprehensive analysis of donor photobleaching data.

Main Methods:

  • Developed new evaluation software for Windows using National Instrument LabVIEW 6.1.

Related Experiment Videos

  • Integrated a mathematical virtual instrument package, including the Levenberg-Marquardt routine.
  • Applied the software to determine FRET efficiency in major histocompatibility complex (MHC) glycoproteins in human B lymphoma cells.

    • Main Results:

    • The software calculates bleaching time constants on a pixel-by-pixel basis.
    • Results can be visualized as color-coded maps or histograms from raw image data.
    • Demonstrated successful application in measuring FRET efficiency for MHC molecules.

    Conclusions:

    • Introduced a new version of pbFRET analysis and data processing software.
    • The software generates a complete analysis pattern for donor photobleaching image series.
    • This tool enhances the capability to study macromolecule interactions using pbFRET.