Recombinant p-hydroxyphenylpyruvate dioxygenase of high activity
1Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, D-78457 Konstanz, Germany.
Zeitschrift Fur Naturforschung. C, Journal of Biosciences
|September 17, 2005
Abstract:
Inhibitors of p-hydroxyphenylpyruvate dioxygenase (HPPD) are bleaching compounds impairing the formation of colored carotenoids. This activity makes them promising candidates for herbicides. Detailed studies on enzyme-inhibitor complexes or on the binding niche of the enzyme have still to be performed. Enzyme preparation from plants is time-consuming and the yield is poor. This paper describes in relevant detail the preparation of recombinant enzyme from Arabidopsis thaliana with good yield and high specific activity.
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