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Updated: Aug 15, 2026

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
Published on: April 5, 2022
Rapid binding of copper(I) to folded aporusticyanin
Luis A Alcaraz1, Antonio Donaire
1Instituto de Biología Molecular y Celular, Universidad Miguel Hernández de Elche, Edificio Torregaitán, Avda. de la Universidad, s/n, 03202 Elche Alicante, Spain.
Abstract:
Kinetics of copper uptake in both oxidation states by the folded and unfolded forms of the type 1 copper protein rusticyanin have been studied. The speed of the binding of copper(I) to the folded rusticyanin is fast, and of the same order of magnitude as copper(I) uptake by the unfolded form. Thus, the binding of copper can be subsequent to the protein folding, contrary to previous proposals. Implications for the mechanism of the formation of the active holoprotein in vivo are discussed.
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