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Molecular cloning and functional expression of d-arabitol dehydrogenase gene from Gluconobacter oxydans in
Hairong Cheng1, Ning Jiang, An Shen
1Center for Microbial Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Abstract:
A NADP-dependent d-arabitol dehydrogenase gene was cloned from Gluconobacter oxydans CGMCC 1.110 and functionally expressed in Escherichia coli. With d-arabitol as sole carbon source, E. coli transformants grew rapidly in minimal medium, and produced d-xylulose. The enzymatic properties of the 29kDa enzyme were documented. The DNA sequence surrounding the gene suggested that it is part of an operon with several components of a sugar alcohol transporter system, and the d-arabitol dehydrogenase gene belongs to the short-chain dehydrogenase family.
