Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Translational deregulation in PDK-1-/- embryonic stem cells.

Yuichi Tominaga1, Tanja Tamgüney, Marina Kolesnichenko

  • 1Cancer Research Institute, University of California, 2340 Sutter St. N319, San Francisco, CA 94115, USA.

Molecular and Cellular Biology
|September 17, 2005
PubMed
Summary

Phosphoinositide-dependent kinase-1 (PDK-1) is essential for mRNA translation in embryonic stem cells. Its absence impairs protein kinase B (PKB) activity, disrupting the translation of key mRNAs.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting NRF2 addiction in cancer: synthetic lethal strategies beyond direct inhibition.

Frontiers in cell and developmental biology·2026
Same author

Site-specific combinatorial ubiquitination drives the targeted degradation of plasma membrane proteins.

bioRxiv : the preprint server for biology·2026
Same author

Rejuvenating the aging gut by targeting senescence.

Nature aging·2026
Same author

Deep learning, deeper relief: pipeline toward tailored analgesia for experimental animal models.

Frontiers in immunology·2025
Same author

Enteric nervous system-derived VIP restrains differentiation of LGR5<sup>+</sup> stem cells toward the secretory lineage impeding type 2 immune programs.

Nature immunology·2025
Same author

Loss of UXS1 Selectively Depletes Pyrimidines and Induces Replication Stress in KEAP1-Mutant Lung Cancer.

Cancer research·2025

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Phosphoinositide-dependent kinase-1 (PDK-1) activates AGC-superfamily kinases via phosphorylation.
  • PDK-1 deficiency reduces protein kinase B (PKB) and p70S6K activity but not mTOR activity.
  • PDK-1 plays a crucial role in cellular signaling pathways.

Purpose of the Study:

  • To investigate the role of PDK-1 in mRNA translation in embryonic stem (ES) cells.
  • To identify specific mRNAs translationally regulated by PDK-1.
  • To elucidate the downstream signaling pathways mediating PDK-1's translational control.

Main Methods:

  • Assessing cellular RNA association with polysomes.
  • Metabolic labeling of nascent proteins.
  • Microarray analysis of total and polysomal RNA from PDK-1 knockout (PDK-1-/-) and wild-type (PDK-1+/+) ES cells.

Related Experiment Videos

Main Results:

  • PDK-1-/- ES cells exhibit significant defects in mRNA translation.
  • Specific mRNAs show decreased or increased association with polysomes in PDK-1 deficient cells.
  • PKB activity, downstream of PDK-1, is critical for the translation of cystatin C, RANKL, and Rab11a mRNAs.

Conclusions:

  • PDK-1 is indispensable for efficient mRNA translation in ES cells.
  • PKB is a key mediator of PDK-1's translational control over specific target mRNAs.
  • mTOR signaling is less critical for the translation of these PDK-1-regulated targets.