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Devosia limi sp. nov., isolated from a nitrifying inoculum.
Bram Vanparys1, Kim Heylen1, Liesbeth Lebbe1
1Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
International Journal of Systematic and Evolutionary Microbiology
|September 17, 2005
Summary
A novel bacterium, Devosia limi, was identified from a nitrifying inoculum. This Gram-negative rod represents a new species within the Devosia genus, distinct from known species.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- Nitrifying inoculums are crucial for nitrogen cycling in various ecosystems.
- The genus Devosia comprises Alphaproteobacteria with diverse metabolic capabilities.
- Accurate bacterial identification is essential for understanding microbial communities and functions.
Purpose of the Study:
- To characterize and classify a newly isolated bacterium from a nitrifying inoculum.
- To determine the taxonomic position of the isolate within the Devosia genus.
- To propose a new species name for the novel bacterial strain.
Main Methods:
- 16S rRNA gene sequencing for phylogenetic analysis.
- DNA-DNA hybridization for genotypic differentiation.
- Fatty acid profiling, SDS-PAGE, and biochemical tests for phenotypic characterization.
Main Results:
- The isolate, designated LMG 22951(T), belongs to the Alphaproteobacteria class.
- Phylogenetic analysis showed relatedness to Devosia neptuniae and Devosia riboflavina.
- Genotypic and phenotypic analyses confirmed LMG 22951(T) as a distinct species within the Devosia genus.
Conclusions:
- The novel bacterium LMG 22951(T) is proposed to be named Devosia limi.
- This finding expands the known diversity of the Devosia genus.
- Devosia limi represents a new member of the Alphaproteobacteria found in nitrifying environments.