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Enterococcus aquimarinus sp. nov., isolated from sea water
Pavel Švec1, Marc Vancanneyt2, Luc A Devriese3
1Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic.
International Journal of Systematic and Evolutionary Microbiology
|September 17, 2005
Summary
Two novel marine enterococcal strains were identified as a new species, Enterococcus aquimarinus. This discovery expands our understanding of enterococci diversity in aquatic environments.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- Enterococci are bacteria found in various environments, including marine ecosystems.
- Accurate bacterial classification is crucial for understanding microbial ecology and potential applications.
Purpose of the Study:
- To taxonomically characterize two enterococcal strains isolated from seawater.
- To determine if these strains represent a previously undescribed species within the Enterococcus genus.
Main Methods:
- Polyphasic taxonomic analysis including protein profiling.
- 16S rRNA gene sequencing for phylogenetic analysis.
- Genomic DNA fingerprinting using (GTG)(5)-PCR and phenylalanyl-tRNA synthase (pheS) gene sequencing.
Main Results:
- The two strains exhibited unique protein profiles distinct from known Enterococcus species.
- 16S rRNA gene sequence analysis showed highest similarity (96.9-96.1%) to Enterococcus saccharolyticus, E. sulfureus, E. saccharominimus, and E. italicus.
- Genomic and biochemical analyses confirmed the strains represent a novel species.
Conclusions:
- The two seawater-derived enterococcal strains are proposed as a new species, Enterococcus aquimarinus sp. nov.
- The type strain for this novel species is LMG 16607(T) (=CCM 7283(T)).
- This finding contributes to the knowledge of bacterial diversity in marine environments.