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Related Experiment Video

Updated: May 5, 2026

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Published on: December 24, 2010

Innovation: Photoactivatable fluorescent proteins.

Konstantin A Lukyanov¹, Dmitry M Chudakov, Sergey Lukyanov

¹Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia. kluk@ibch.ru

Nature Reviews. Molecular Cell Biology

|September 17, 2005

Summary

This summary is machine-generated.

Photoactivatable fluorescent proteins offer controllable optical labeling for tracking cells and molecules. Their unique properties enable precise spatio-temporal imaging in live biological systems.

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Area of Science:

Biochemistry
Cell Biology
Microscopy

Background:

Photoactivatable fluorescent proteins (PA FPs) are crucial tools in modern biological research.
Controlling fluorescence properties via light allows for advanced imaging techniques.

Purpose of the Study:

To review the properties of currently available photoactivatable fluorescent proteins.
To explore the potential applications of these proteins in biological research.

Main Methods:

Discussion of the photophysical properties of various PA FPs.
Review of literature on PA FP applications in live-cell imaging.

Main Results:

PA FPs exhibit tunable fluorescence characteristics based on light stimuli.
These proteins facilitate spatio-temporal optical labeling and tracking of cellular components.

Conclusions:

Photoactivatable fluorescent proteins provide powerful capabilities for advanced biological imaging.
Further development and application of PA FPs will enhance our understanding of dynamic biological processes.