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Conjugation of fluorophores to tubulin

John Peloquin1, Yulia Komarova, Gary Borisy

  • 1Department of Cell and Molecular Biology, Northwestern University Medical School, 303 E. Chicago Ave., Chicago, Illinois 60611, USA.

Nature Methods
|September 20, 2005
PubMed
Abstract

No abstract available in PubMed .

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There are three types of cytoskeletal structures in eukaryotic cells—microfilaments, intermediate filaments, and microtubules. With a diameter of about 25 nm, microtubules are the thickest of these fibers. Microtubules carry out a variety of functions that include cell structure and support, transport of organelles, cell motility (movement), and the separation of chromosomes during cell division.Microtubules are hollow tubes whose walls are made up of globular tubulin proteins. Each tubulin...