Related Experiment Video

Updated: Aug 15, 2026

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

The expression of the ubiquitin ligase subunit Cks1 in human breast cancer

Merav Slotky1, Ma'anit Shapira, Ofer Ben-Izhak

  • 1Department of Surgery A, Rambam Medical Center and the Technion-Israel Institute of Technology, Haifa, Israel. m_slotky@rambam.health.gov.il

Breast Cancer Research : BCR
|September 20, 2005
PubMed
Abstract

Insights

Cyclin-dependent kinase subunit 1 (Cks1) overexpression in breast cancer correlates with poor prognosis by decreasing the cell-cycle inhibitor p27Kip1. Cks1 may drive aggressive tumor behavior and warrants further investigation.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Loss of p27Kip1, a cell-cycle inhibitor, is linked to poor breast cancer prognosis.
  • p27Kip1 degradation is regulated by Skp2 and Cks1, its ubiquitin ligase subunits.
  • Skp2 is overexpressed in breast cancer, but Cks1's role remains unclear.

Purpose of the Study:

  • To investigate the role of Cks1 expression in breast cancer.
  • To examine the relationship between Cks1, p27Kip1, and Skp2 expression.
  • To determine Cks1's association with tumor aggressiveness.

Main Methods:

  • Immunohistochemistry and immunoblot analysis of Cks1, Skp2, and p27Kip1 expression in patient tissues and cell lines.
  • Statistical analysis using Cox regression and Kaplan-Meier methods to correlate Cks1 levels with clinical parameters.

Main Results:

  • Cks1 expression strongly correlated with Skp2 and inversely with p27Kip1.
  • Cks1 overexpression was linked to poor tumor differentiation, younger age, loss of hormone receptors, and reduced survival.
  • Estradiol increased Cks1/Skp2, while tamoxifen decreased them in cell lines.

Conclusions:

  • Cks1 contributes to p27Kip1 downregulation in breast cancer.
  • Cks1 plays a significant role in the development of aggressive breast cancer phenotypes.
  • Targeting Cks1 may offer a therapeutic strategy for aggressive breast cancer.

More Related Videos

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

Comparative Strategies for Ubiquitination Detection in Mammalian Cell Lysates Using SMAD2/SMURF2 as a Model09:00

Comparative Strategies for Ubiquitination Detection in Mammalian Cell Lysates Using SMAD2/SMURF2 as a Model

Published on: April 17, 2026

Related Concept Videos

Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...