Production of hydrogen peroxide in rat corpus cavernosum: an on-line study with microvoltammetric electrodes

Continuous measurements were realized in rat corpus cavernosum using microvoltammetric captor. After inhibition of endothelial NO-synthase by L-NMA vasodilatation was induced by intracavernous injection of acetylcholine. Blood flow of rat penis was monitored by laser Doppler. A new peak appeared in reduction. It was identified as hydrogen peroxide by its peak potential, by intracavernous injection of hydrogen peroxide or catalase. Intracavernous injection of various pharmacological agents permitted to demonstrate that its origin was due to endothelial NAD(P)H oxidases. DPI inhibited its synthesis; NADH and NADPH enhanced it. Intracavernous injection of diethyldithiocarbamic acid gave the disappearance of hydrogen peroxide peak and appearance of the superoxide peak. This preliminary study showed that when the L-arginine pathway was inhibited, the NAD(P)H oxidase pathway functioned in rat corpus cavernosum for vasodilatation.