Related Experiment Video
Updated: Jan 19, 2026
A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis
Published on: March 24, 2017
Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase
Manoj Kumar1, Inshad Ali Khan, Vijashoor Verma
1Biotechnology Division, Regional Research Laboratory, Jammu-Tawi 180001, India.
Abstract:
A rapid colorimetric, microplate-based nitrate reductase assay (NRA) method for antibiotic susceptibility profile of clinical isolates of Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportion method. The overall agreement between the results obtained by NRA and ABA was 99% and 98%, respectively, when compared with agar proportion method. Reproducible results for all the isolates were obtained within 8 days by NRA as well as ABA. The specificity of NRA method for M. tuberculosis makes it more suitable method for MIC determination.
More Related Videos
15:28A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
Published on: January 17, 2014
12:39Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
Published on: June 24, 2011