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Updated: Aug 15, 2026

Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
Expression of a novel Ski-like gene in Xenopus development
Daniel W Seufert1, Rashmi S Hegde, Srivamsi Nekkalapudi
1Center for Molecular and Human Genetics, Columbus Children's Research Institute, 700 Children's Dr., Columbus, OH 43026, USA.
Abstract:
Members of the Ski/Sno family of gene products contain a characteristic peptide domain involved in protein-protein or protein-DNA interaction. Here, we characterize the developmental expression of xDawg, in Xenopus laevis, of a new gene, related to the Ski/Sno family of transcription regulators. The Ski/Sno domain of xDawg is predicted to present an electropositive surface, consistent with a role in DNA binding. This gene is expressed in the marginal zone of early gastrulae, and in the brain, sensory vesicles, and cranial neural crest of neurula and tailbud embryos.
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