Related Experiment Video

Updated: Aug 15, 2026

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
08:33

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Published on: March 11, 2021

Role of aromatic localization in the gating process of a potassium channel

Carmen Domene, Satyavani Vemparala, Michael L Klein

    Biophysical Journal
    |September 20, 2005
    PubMed

    More Related Videos

    Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels11:19

    Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

    Published on: July 4, 2016

    Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors10:59

    Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

    Published on: February 10, 2014

    Related Concept Videos

    Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

    Ligand-Gated Ion Channel Receptor: Gating Mechanism

    Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
    Non-gated Ion Channels01:24

    Non-gated Ion Channels

    Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
    Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
    Non-gated Ion Channels01:24

    Non-gated Ion Channels

    Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
    Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
    Ligand-gated Ion Channels01:19

    Ligand-gated Ion Channels

    Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
    Three Subfamilies of Ligand-gated Ion Channels
    Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
    Ligand-gated Ion Channels01:19

    Ligand-gated Ion Channels

    Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
    Three Subfamilies of Ligand-gated Ion Channels
    Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
    Voltage-gated Ion Channels01:26

    Voltage-gated Ion Channels

    Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
    Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...