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Culture and Maintenance of Human Embryonic Stem Cells
09:36

Culture and Maintenance of Human Embryonic Stem Cells

Published on: December 22, 2009

Culture of human embryonic stem cells

Gerald Schatten1, Joseph Smith, Christopher Navara

  • 1Pittsburgh Development Center, Department of Obstetrics, Gynecology & Reproductive Sciences, Division of Developmental and Regenerative Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA. gschatten@pdc.magee.edu

Nature Methods
|September 21, 2005
PubMed
Abstract

No abstract available in PubMed .

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Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

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