Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mucosal immunity induced by pneumococcal glycoconjugate.

Chi-Jen Lee1, Lucia H Lee, Xin-Xing Gu

  • 1Center for Biologics and Research, Food and Drug Administration, Rockville, MD 20852, USA. lee_chi@cber.fda.gov

Critical Reviews in Microbiology
|September 21, 2005
PubMed
Summary

Intranasal immunization with a pneumococcal glycoconjugate vaccine, enhanced by CpG oligodeoxynucleotide (ODN) adjuvant, significantly boosted antibody responses. This suggests a promising strategy for developing effective mucosal vaccines against Streptococcus pneumoniae infections.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Overcoming Waning Immunity in Pertussis Vaccines: Workshop of the National Institute of Allergy and Infectious Diseases.

Journal of immunology (Baltimore, Md. : 1950)·2020
Same author

Waning Immunity and Microbial Vaccines-Workshop of the National Institute of Allergy and Infectious Diseases.

Clinical and vaccine immunology : CVI·2017
Same author

Towards New Broader Spectrum Pneumococcal Vaccines: The Future of Pneumococcal Disease Prevention.

Vaccines·2015
Same author

Late acyltransferase genes lpxX and lpxL jointly contribute to the biological activities of Moraxella catarrhalis.

Journal of medical microbiology·2013
Same author

Moraxella catarrhalis activates murine macrophages through multiple toll like receptors and has reduced clearance in lungs from TLR4 mutant mice.

PloS one·2012
Same author

Role of c-Jun N-terminal protein kinase 1/2 (JNK1/2) in macrophage-mediated MMP-9 production in response to Moraxella catarrhalis lipooligosaccharide (LOS).

PloS one·2012

Area of Science:

  • Immunology
  • Vaccinology
  • Microbial Pathogenesis

Background:

  • Host defenses against Streptococcus pneumoniae rely on opsonophagocytosis via antibodies and complement.
  • Mucosal immunity, involving secretory IgA, is crucial for defending against respiratory pathogens like S. pneumoniae.
  • Mucosal immunization strategies are being developed, with CpG oligodeoxynucleotide (ODN) showing promise as an adjuvant.

Purpose of the Study:

  • To investigate the efficacy of intranasal immunization using a pneumococcal glycoconjugate vaccine combined with CpG ODN as a mucosal adjuvant.
  • To evaluate the induction of specific antibody responses in mice following mucosal immunization.

Main Methods:

  • Mice were immunized intranasally with a type 9V polysaccharide (PS) conjugated to inactivated pneumolysin (Ply), with or without CpG ODN.

Related Experiment Videos

  • Antibody levels (IgG, IgA, and IgG subclasses) in sera were measured and compared between groups.

    • Main Results:

    • Immunization with 9V PS-Ply conjugate plus CpG ODN induced significantly higher levels of 9V PS-specific IgG and IgA antibodies compared to the conjugate alone.
    • High levels of IgG1, IgG2, and IgG3 subclasses were observed in mice receiving the conjugate plus CpG ODN.
    • Elevated IgG and IgA responses were also noted in young mice immunized with the conjugate plus CpG or non-CpG adjuvant.

    Conclusions:

    • Mucosal immunization with pneumococcal glycoconjugate vaccines using CpG ODN as an adjuvant effectively induces protective immune responses.
    • This approach holds potential for developing novel vaccines against Streptococcus pneumoniae infections, particularly targeting mucosal surfaces.