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Updated: Aug 15, 2026
Prostaglandin Extraction and Analysis in Caenorhabditis elegans
Published on: June 25, 2013
LC-MS/MS-analysis of prostaglandin E2 and D2 in microdialysis samples of rats
Ronald Schmidt1, Ovidiu Coste, Gerd Geisslinger
1pharmazentrum frankfurt/ZAFES/Institut für Klinische Pharmakologie, Klinikum der Johann Wolfgang Goethe-Universität Frankfurt, Theodor Stern Kai 7, Haus 75/4.OG, 60590 Frankfurt, Germany. ronald.schmidt@em.uni-frankfurt.de
Abstract:
For the determination of prostaglandins in microdialysis samples, usually immunoassays are used. However, these assays may show cross-reactivity among various prostaglandins. To overcome this problem a specific method for the determination of prostaglandin E2 and D2 in rat microdialysis samples by using liquid chromatography-electrospay ionization-tandem mass spectrometry (LC-ESI-MS/MS) is described. Prostaglandin E2 and D2 were extracted from microdialysis samples with liquid-liquid extraction using deuterated prostaglandin D2, [2H4]-PGD2, as internal standard. Subsequently, prostaglandins were separated with a phenomenex Synergi Hydro-RP column and determined with a PE Sciex API 3000 mass spectrometer equipped with a turbo ion spray interface operating in negative ionization mode. The method showed a LLOQ of 25 pg/ml for prostaglandin E2 and 50 pg/ml for prostaglandin D2. The applicability of the method is shown in rat spinal cord microdialysis samples following peripheral nociceptive stimulation.
