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Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats
Published on: January 31, 2019
Necrophysiological determination of blood pressure in fishes
David R Jones1, Kevina Perbhoo, Marvin H Braun
1Zoology Animal Care, University of British Columbia, 6199 South Campus Road, Vancouver, BC, V6T 1W5, Canada. jones@zoology.ubc.ca
Abstract:
Bony fishes have an elastic chamber between the heart and aorta, the bulbus arteriosus, which has unique mechanical properties. On inflation, the isolated bulbus is initially very stiff but soon becomes extremely compliant yielding a steady (plateau) pressure upon further inflation, which appears to be similar in any given species. Here we show that the plateau pressure correlates with mean blood pressure determined in vivo. Consequently, inflation of the bulbus can be used to determine blood pressure in the living animal from recordings made after it is dead.
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This invasive approach involves cannulating a peripheral artery. During each cardiac contraction, pressure generates mechanical motion within the catheter, transmitted through rigid, fluid-filled tubing to a transducer. This transducer converts mechanical motion into electrical signals displayed as waveforms on a monitor. An automatic flushing system prevents blood backflow. Due to the potential risk of unexpected arterial blood loss, this method is primarily used in intensive...
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