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Molecular study of Echinococcus in west-central China
Y R Yang1, M C Rosenzvit, L H Zhang
1Molecular Parasitology Laboratory, Queensland Institute of Medical Research, 300 Herston Road, Brisbane, Q 4006, Australia.
Parasitology
|September 22, 2005
Summary
Genetic analysis of Echinococcus parasites in West-central China reveals the predominant G1 genotype of Echinococcus granulosus in various hosts. Some genetic variations were observed, potentially impacting disease control and treatment strategies for echinococcosis.
Area of Science:
- Veterinary Parasitology
- Molecular Epidemiology
- Infectious Diseases
Background:
- West-central China is a significant region for alveolar and cystic echinococcosis.
- Echinococcus granulosus and Echinococcus multilocularis infect various intermediate hosts in this area.
Purpose of the Study:
- To genotype Echinococcus granulosus and Echinococcus multilocularis isolates from West-central China.
- To investigate genetic variations within these parasite populations.
- To assess the potential impact of genetic diversity on echinococcosis epidemiology and control.
Main Methods:
- Collection of Echinococcus granulosus isolates from humans and animals (sheep, cattle, goats, yaks) in Qinghai, Ningxia, Gansu, and Sichuan.
- Genotyping using the mitochondrial DNA ATP synthase subunit 6 gene (atp6).
- Sequence analysis of E. multilocularis isolates.
Main Results:
- The sheep strain (G1 genotype) of E. granulosus was the only genotype found in sheep, cattle, goats, yaks, and humans.
- Heterogeneity in the atp6 sequence was observed in some E. granulosus isolates, with a common silent substitution (G/A) at position 360.
- Two E. multilocularis isolates showed sequence variations from each other and the reference sequence.
Conclusions:
- The identified genotypic variations in Echinococcus parasites may lead to phenotypic differences.
- These variations could influence host infectivity, drug sensitivity, and virulence.
- Understanding genetic diversity is crucial for effective echinococcosis control, vaccination, and treatment strategies.