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Effects of propyl gallate on interaction between TNF-alpha and sTNFR-I using an affinity biosensor
Jing Li1, Jia-dong Huang, Bo-yan Wu
1Laboratory of Biosensors, College of Life Science, Nankai University, Tianjin 300071, China.
Aim:
To study the effects of propyl gallate on the interaction of tumor necrosis factor-alpha (TNF-alpha) with its soluble receptor, sTNFR-I.
Methods:
Interactions between TNF-alpha and sTNFR-I were analyzed using an IAsys biosensor. sTNFR-I was immobilized on the carboxymethyl dextran (CMD) surface of the IAsys biosensor cuvettes, and TNF-alpha preincubated with different concentrations of propyl gallate was added to the cuvettes. The resonant angle shift caused by the binding between TNF-alpha and sTNFR-I was then recorded.
Results:
sTNFR-I was immobilized on the CMD surface at a density of 2.76 ng/mm(2). TNF-alpha then bound the immobilized sTNFR-I specifically, and propyl gallate was able to enhance the binding between TNF-alpha and sTNFR-I in a dose-dependent manner.
Conclusion:
The binding between TNF-alpha and sTNFR-I is one of the targets that propyl gallate can act on in vivo. The IAsys biosensor offers a new clue as to the study on the mechanisms of action of propyl gallate.
