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Updated: Aug 14, 2026
An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
Published on: May 4, 2021
[Application of green fluorescent protein gene in study of adipocyte differentiation]
Ji Zhu1, Wen-li Ma, Xiang-ming Mao
1Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China.
Objective:
To establish a model for dynamic detection of adipocyte differentiation in vivo by green fluorescent protein (GFP).
Methods:
The promoter of peroxisome proliferators-activated receptor gamma 2 (PPARgamma2) gene was amplified by PCR from the genomic DNA of human preadipocytes, inserted into the eukaryotic expression vector pEGFP-1 and transferred into NIH3T3 fibroblasts and preadipocytes which were induced to differentiate into adipocyte.
Results:
Upon induction with insulin, dexamethasone and 3-isobutyl-1-methyl xanthine, the transformed human preadipocytes differentiated into mature adipocytes and expressed GFP, but NIH3T3 fibroblasts could not differentiate into adipocytes or express GFP.
Conclusion:
Transformation of human preadipocytes with the recombinant of PPARgamma2 promoter with GFP gene can be employed to dynamically detect the differentiation of adipocytes in vivo.
