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Related Experiment Videos

A resistive-pulse sensor chip for multianalyte immunoassays.

A Carbonaro1, L L Sohn

  • 1Department of Mechanical Engineering, University of California, Berkeley, CA 94720, USA.

Lab on a Chip
|September 22, 2005
PubMed
Summary

This study introduces a novel chip with multiple pores for simultaneous detection of multiple antigens using resistive-pulse sensing. This scalable technology enables rapid, multiplexed diagnostics for various pathologic conditions.

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Biological sensing with an on-chip resistive pulse analyzer.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference·2007

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Multi-analyte immunoassays are crucial for diagnosing complex pathologic conditions.
  • Current methods can be time-consuming and require multiple tests.

Purpose of the Study:

  • To develop a single-chip solution for rapid, simultaneous detection of multiple antigens.
  • To demonstrate the feasibility of integrating resistive-pulse sensing with multiple artificial pores.

Main Methods:

  • Integration of resistive-pulse sensing with multiple artificial pores on a single chip.
  • Utilizing latex colloids functionalized with antibodies to capture specific antigens.
  • Detecting antigen-antibody binding via changes in colloid size measured by pore resistance.

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Main Results:

  • Successful simultaneous detection of human G-CSF and GM-CSF antigens on a single chip.
  • Demonstrated proof-of-principle for multiplexed antigen detection.
  • Validated the size-change detection mechanism of antibody-bound colloids.

Conclusions:

  • The developed multi-pore resistive-pulse sensing chip offers a scalable platform for multiplexed antigen detection.
  • This technology has the potential to significantly advance rapid and simultaneous diagnostic capabilities.
  • Future iterations can be scaled to detect numerous antigens using an N x N pore array.