Biocompatibility parameters in in-vitro simulated automated versus continuous ambulatory peritoneal dialysis

Area of Science:

• Nephrology
• Biocompatibility Studies
• Cell Culture Models

Background:

• Automated peritoneal dialysis (APD) use is increasing.
• APD involves larger fluid volumes and more frequent dialysate contact.
• Potential for increased negative impact on biocompatibility compared to conventional peritoneal dialysis (PD) fluids.

Purpose of the Study:

• To compare the biocompatibility of APD and continuous ambulatory peritoneal dialysis (CAPD) using an in-vitro model.
• To assess the acute effects of APD and CAPD on human mesothelial cells.

Main Methods:

• A double chamber cell culture system with human mesothelial cells was used.
• Mimicked eight-hour CAPD and CCPD (continuous cycler-assisted peritoneal dialysis) settings with conventional PD solution.
• Assessed cell viability (MTT assay), IL-6 release, CA125, and TGF-β1.

Main Results:

• Both CAPD and CCPD modes showed reduced MTT assay and stimulated IL-6 release compared to control.
• No significant differences were observed in TGF-β1 and CA125 release between PD modes and control.
• CAPD and CCPD modes did not differ in MTT assay, IL-6, TGF-β1, or CA125 generation.

Conclusions:

• The in-vitro model suggests APD does not have an acute, additive negative impact on biocompatibility.
• APD did not negatively affect peritoneal host defense, mesothelial cell integrity, or fibrosis induction.
• Findings indicate APD is comparable to CAPD regarding acute biocompatibility.