Related Experiment Video
Updated: Aug 15, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Screening for anabolic steroids in doping analysis by liquid chromatography/electrospray ion trap mass spectrometry
K Deventer1, P Van Eenoo, F T Delbeke
1Ghent University (UGent), Department of Clinical Chemistry, Microbiology and Immunology, Doping Control Laboratory (DoCoLab), Technologiepark 30, B-9052 Zwijnaarde, Belgium. Koen.Deventer@UGent.be
Abstract:
A fast and selective LC/MS/MS method for the screening of four anabolic steroids in human urine has been developed and validated. Liquid-liquid extraction with diethyl ether was applied after enzymatic hydrolysis. Analyses were performed on an ion trap mass spectrometer equipped with electrospray ionisation. MS/MS was applied for all compounds. The analytical run time was 11 min. The LOD for all compounds varied between 1 and 10 ng/mL. Left-over A samples, which were declared positive by GC/MS for the presence of 3'-hydroxystanozolol, were assessed using the described method.
Related Concept Videos
High-Performance Liquid Chromatography: Types of Detectors
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Ion-Exchange Chromatography

