Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Programmable assembly of nanoarchitectures using genetically engineered viruses.

Yu Huang1, Chung-Yi Chiang, Soo Kwan Lee

  • 1Department of Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

Nano Letters
|September 24, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Laser-Induced Creation of Coherent V2 Centers in Bulk-Grown Silicon Carbide.

Nano letters·2026
Same author

Controlling metal-carbonate phase, form, and function through de novo protein design.

bioRxiv : the preprint server for biology·2026
Same author

Nanofiber Length Shapes Vaccine Immunity: Mechanistic Insights from M13 Bacteriophage.

Nano letters·2026
Same author

In Situ Crosslinking of Bioorthogonal Nanoparticles to Restore Clot Stability in Coagulopathic Blood.

Bio-protocol·2025
Same author

De novo design of metal-oxide templating proteins.

bioRxiv : the preprint server for biology·2025
Same author

A Suspended 4H-Silicon Carbide Membrane Platform for Defect Integration into Quantum Devices.

Nano letters·2025

Engineered M13 bacteriophage (phage) viruses were used as templates to precisely assemble nanoparticles into complex nanostructures like arrays and nanowires. This programmable self-assembly offers a novel method for creating advanced nanomaterials.

Area of Science:

  • Biomaterials Engineering
  • Nanotechnology
  • Molecular Biology

Background:

  • Biological systems exhibit natural molecular recognition and self-assembly.
  • These capabilities are attractive for creating complex materials with molecular precision.
  • M13 bacteriophage (phage) are filamentous viruses that can be engineered.

Purpose of the Study:

  • To engineer M13 phage for programmable nanoparticle assembly.
  • To create diverse nanostructures including nanoparticle arrays and nanowires.
  • To demonstrate the utility of engineered phage as a generic templating system.

Main Methods:

  • Rational engineering of the M13 phage genome to express specific peptides.
  • Displaying gold-binding and streptavidin-binding motifs on phage particles.

Related Experiment Videos

  • Utilizing engineered phage to assemble gold (Au) and cadmium selenide (CdSe) nanocrystals.
  • Using assembled nanoparticle arrays to nucleate nanowire formation.

    • Main Results:

    • Successfully created M13 phage clones with specific binding motifs.
    • Assembled Au and CdSe nanocrystals into ordered 1D arrays and complex geometries.
    • Demonstrated that nanoparticle arrays can template conductive nanowire growth.
    • Engineered phage serve as a versatile platform for programmable nanostructure assembly.

    Conclusions:

    • Engineered M13 phage provide a powerful and programmable platform for nanoscale construction.
    • This method enables precise assembly of various nanostructures with potential applications in electronics.
    • The phage-based templating approach facilitates the creation of complex, functional nanomaterials.