Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolation and characterization of a mouse subtelomeric sequence.

D Broccoli1, O J Miller, D A Miller

  • 1Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, MI 48202.

Chromosoma
|April 1, 1992
PubMed
Summary

Researchers identified a novel mouse subtelomeric DNA sequence, ST1, using polymerase chain reaction (PCR). This sequence is located at mouse telomeres and shows a specific orientation relative to minor satellite sequences.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Large-scale single-molecule analysis of tau proteoforms.

bioRxiv : the preprint server for biology·2025
Same author

Cadmium phytoextraction by Helianthus annuus (sunflower), Brassica napus cv Wichita (rapeseed), and Chyrsopogon zizanioides (vetiver).

Chemosphere·2020
Same author

A Multicenter Pilot Study on the Clinical Utility of Computational Modeling for Flow-Diverter Treatment Planning.

AJNR. American journal of neuroradiology·2019
Same author

Demographic and mortality analysis of hospitalized children at a referral hospital in Addis Ababa, Ethiopia.

BMC pediatrics·2016
Same author

Prenatal diagnosis of intraventricular hemorrhage in a fetus with congenital diaphragmatic hernia using magnetic resonance imaging.

Minerva ginecologica·2015
Same author

Psychiatry clerkship sites : factors that make a difference in student performance.

Academic psychiatry : the journal of the American Association of Directors of Psychiatric Residency Training and the Association for Academic Psychiatry·2014

Area of Science:

  • Genetics
  • Molecular Biology
  • Genomics

Background:

  • Telomeres are protective caps at the ends of eukaryotic chromosomes.
  • Subtelomeric regions contain repetitive DNA sequences with regulatory functions.
  • Understanding subtelomeric sequences is crucial for comprehending genome stability and evolution.

Purpose of the Study:

  • To identify and characterize novel subtelomeric DNA sequences in mice.
  • To determine the genomic location and organization of the identified sequence.
  • To investigate the relationship between the novel sequence and other repetitive elements like minor satellite DNA.

Main Methods:

  • Polymerase chain reaction (PCR) using telomeric primers to amplify subtelomeric DNA.
  • Cloning and sequencing of the amplified DNA fragment (ST1).

Related Experiment Videos

  • Southern analysis and in situ hybridization to confirm genomic presence and chromosomal mapping.
  • PCR experiments with combinations of primers for telomeric, minor satellite, and ST1 sequences.

    • Main Results:

    • A novel 670 bp mouse subtelomeric sequence, ST1, was identified and cloned.
    • ST1 is flanked by inverted telomeric hexanucleotide repeats (TTAGGG)n.
    • ST1 is present in mouse genomic DNA and maps to most, if not all, mouse telomeres.
    • ST1 sequences are sometimes adjacent to minor satellite sequences with a specific orientation.

    Conclusions:

    • ST1 represents a novel component of the mouse subtelomeric region.
    • The specific localization and orientation of ST1 suggest a potential role in telomere structure or function.
    • The association with minor satellite sequences provides insights into the organization of complex repetitive DNA at chromosome ends.