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Related Experiment Videos

Weak D type 1.1 exemplifies another complexity in weak D genotyping.

Andrea Doescher1, Willy A Flegel, Eduard K Petershofen

  • 1DRK Blutspendedienst NSTOB, Oldenburg Institute, Oldenburg, Germany.

Transfusion
|September 27, 2005
PubMed
Summary
This summary is machine-generated.

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A new weak D type 1.1 allele was identified in Northern Germany, causing misidentification of blood types. This highlights issues in current RhD genotyping, including allele mutations and geographic variations in distribution.

Area of Science:

  • Transfusion Medicine
  • Genetics
  • Immunology

Background:

  • Weak D expression in red blood cells results from numerous RHD alleles.
  • Polymerase chain reaction (PCR) is increasingly used to identify RHD alleles for transfusion decisions.
  • Rare D variants can be misidentified as common weak D types, posing diagnostic challenges.

Purpose of the Study:

  • To investigate a novel weak D variant, RHD(L18V,V270G), termed weak D type 1.1.
  • To evaluate the diagnostic accuracy of current RhD genotyping methods.
  • To assess the serologic characteristics and prevalence of weak D type 1.1.

Main Methods:

  • Weak D donors were analyzed using direct column agglutination.
  • Molecular typing involved weak D PCR and nucleotide sequencing for unusual D expression.

Related Experiment Videos

  • Serologic profiling utilized agglutination and flow cytometry.

    • Main Results:

    • The RHD(L18V,V270G) allele, weak D type 1.1, was identified in donors with minimal D expression.
    • These donors were initially misclassified as weak D type 1 by PCR.
    • Weak D type 1.1 was found in 7 of 23 donors in a specific region of Northern Germany and possesses approximately 600 D antigens per red blood cell.

    Conclusions:

    • The distinct phenotype of weak D type 1.1 reveals limitations in current RhD genotyping strategies.
    • Mistyping can occur due to additional mutations within RHD alleles.
    • Significant geographic variations in RHD allele distribution exist.