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Polyclonal antibody secretion induced in human mixed lymphocyte cultures
Scandinavian Journal of Immunology
|January 1, 1979
Summary
Human spleen cells and lymphocytes in mixed leucocyte culture (MLC) secreted antibodies, identified as IgM, IgG, and IgA. Antibody secretion peaked on day 5 and showed no correlation with DNA synthesis.
Area of Science:
- Immunology
- Cell Biology
Background:
- Mixed leucocyte culture (MLC) is a model for studying immune responses.
- Antibody secretion is a key function of B lymphocytes.
Purpose of the Study:
- To investigate antibody secretion in human mixed leucocyte culture (MLC).
- To characterize the types of antibodies secreted during MLC.
- To explore the relationship between antibody secretion and DNA synthesis in MLC.
Main Methods:
- Human spleen cells and thoracic duct lymphocytes were cultured in MLC.
- Antibody-secreting cells were quantified using a haemolysis-in-gel assay (plaque-forming cells, PFC).
- Fluorescein isothiocyanate (FITC)-coupled and protein-A-coupled sheep erythrocytes (SRBC) were used as targets.
Main Results:
- Peak antibody secretion was observed on day 5 of MLC.
- Secreted antibodies were identified as IgM, IgG, and IgA.
- No correlation was found between plaque-forming cells (PFC) and DNA synthesis.
- MLC supernatants did not induce antibody secretion.
Conclusions:
- Human MLC can induce antibody secretion by lymphocytes.
- The secreted antibodies are of multiple immunoglobulin classes (IgM, IgG, IgA).
- Antibody production in MLC is distinct from DNA synthesis and cell proliferation.