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Detection of elements responsible for stage- and tissue-specific expression of mouse Sry using an in vitro Cre/loxP

Masanori Ito1, Kou Yokouchi, Kunihiko Naito

  • 1Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. mito@tmh.tmc.edu

Biochemical and Biophysical Research Communications
|September 27, 2005
PubMed

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...